Aliases
CREB2; CREBP1;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P15336
NCBI Protein: NP_001871.2
Immunogen
Peptide sequence around phosphorylation site of threonine 73 or 55 (T-P-T(p)-R-F) derived from Human ATF2.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat Monkey
Tested Applications
WB IHC IF Recommended dilution: Predicted MW: 65-75 kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background / Function
Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5′-GTGACGT[AC][AG]-3′), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex. Beausoleil S A, et al. (2004) Proc Natl Acad Sci U S A. 101(33): 12130-12135. Abdel-Hafiz H A, et al. (1992) Mol Endocrinol. 6: 2079-2089. Gupta S, et al. (1995) Science. 267: 389-393. Van Dam H, et al. (1995) EMBO J. 14(8): 1798-1811. Livingstone C, et al. (1995) EMBO J. 14: 1785-1797.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Thr73 or 55
