Aliases
Ataxia telangiectasia mutated homolog; Ataxia telangiectasia mutated; kinase ATM
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q13315
NCBI Protein: NP_000042.3
Immunogen
Peptide sequence around aa.1979~1983 (E-G-S-Q-S) derived from Human ATM.
Raised In
Rabbit
Species Reactivity
Human Mouse
Tested Applications
WB IF Recommended dilution: Predicted MW: 350kd, Western blotting: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Background / Function
ATM encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene.Gupta A. et al. (2005) Mol Cell Biol. 25(12): 5292-5305.Bernstein JL. et al. (2002) Breast Cancer Res. 4(6): 249-252.Silverman J. et al. (2004) Genes Dev. 18(17): 2108-2119.Nakada D. et al. (2003) Nucleic Acids Res. 31(6): 1715-1724.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
