Description
Product Description
Avidin has a very high affinity for biotin molecule, one million times than the common affinity between antigen and antibody. Avidin is an alkalic protein (IP=10.0-10.5), and it can transfer to be a neutral protein through reconstruction. It is a glycosylated biotin-binding protein that has a basic isoelectric point and is widely used to detect biotinylated probes.
Avidin is a 65 kDa protein found in egg whites. It consists of 4 identical subunits, each with a high-affinity binding site for biotin (Vitamin H). The strength of the binding (Ka = 1015) results in an essentially irreversible interaction. This interaction has been exploited for immuno-labeling of antigens in histochemical, blotting, and multiwell assays. Biotinylated probes, which may be secondary antibodies, lectins, or other bioactive compounds, bind to targets on tissue samples, microtiter plates, or membranes. Avidin conjugated to enzymes, fluorochromes, or other labels binds to the biotinylated probes for visualization, either by detection of fluorescence or enzymatic conversion of substrate to produce a visible end product. In this system avidin serves as a secondary probe, attaching to the primary biotinylated probe at several sites and amplifying the signal. Use of an avidin-enzyme conjugate provides further amplification by conversion of substrate by the enzyme, which will continue to produce a visible product until the substrate is exhausted or the reaction is stopped.
Avidin has been reported to exhibit non-specific binding to membranes and tissues.
FITC conjugated monoclonal anti-avidin may be used for single or double immunofluorescence labeling techniques and for modification of non-fluorescent to fluorescent reactions.
Conjugate: FITC
Form: Concentrated, Liquid
Concentration: 1mg/ml
Storage: At 4˚C for one year.
Application: IHC-P, IHC-F, ICC, FCM
Application details
Flowcytometry|10μg/ml|Immunohistochemistry(Paraffin-embedded Section)|15.6μg/ml|Immunohistochemistry(Frozen Section)|15.6μg/ml|Immunocytochemistry|15.6μg/ml|
Additional information
| Size | 0.5ml, 1ml |
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