Description
Product Description
Avidin is a 68KD protein that extracted from egg whites. It has very high affinity to biotin molecule, one million times than the common affinity between antigen and antibody. Avidin is a alkalic protein (IP=10.0-10.5), and it can transfer to be a neutral protein through reconstruction.
Since Avidin binds strongly to biotin, thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.
Using biotinylated primary or secondary intermediates and peroxidase-labeled Avidin D, antigens can be localized in histological sections, cytospin preparations, or smears.
Avidin-Peroxidase has been used for detecting anti-5-bromodeoxyuridine (anti-BrdU) binding and OVA-IgG1.
To detect Ga1-deficient IgA1 antibodies in mouse serum, ELISA assays were performed using sera which was incubated with biotinylated HAA, which binds IgA1, in 96-well plates. HRP-conjugated avidin was then incubated with the sera for 40 minutes at 37 degrees.
Conjugate: HRP
Form: Concentrated, Liquid
Concentration: 1mg/ml
Storage: At 4˚C for one year.
Application: Dotblot, WB
Application details: Dot blot(ECL)|0.25-0.5μg/ml|Western blot(DAB)|0.3-2μg/ml|Western blot(ECL)|0.1-0.3μg/ml|
Additional information
| Size | 0.5ml, 1ml |
|---|
