Aliases
B-RAF; RAFB1; RMIL; BRAF1; c-RMIL
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot#: P15056;
NCBI Gene#: 673;
NC
Immunogen
Peptide sequence around phosphorylation site of threonine 599 (L-A-T(p)-V-K) derived from Human B-RAF.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
IHC Recommended dilution: Immunohistochemistry: 1:50~1:100
Background / Function
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.Armelle Calipel, J. Biol. Chem., Oct 2003; 278: 42409 – 42418. Tara J. Dillon, J. Biol. Chem., Sep 2003; 278: 35940 – 35949. R K Jaiswal, Mol. Cell. Biol., Oct 1994; 14: 6944 – 6953. Laura L. Dugan, J. Biol. Chem., Sep 1999; 274: 25842.
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Thr599
