General Description
Bicinchoninic acid (BCA) is commonly used for the determination of protein concentration as well as a photometric reagent for protein assay. It acts by developing an intense purple complex with the cuprous ion under alkaline conditions, and hence provides a platform for the analytical monitoring of cuprous ion produced in the biuret reaction. BCA displays increased tolerance against interference from non-ionic detergents and simple buffer salts.
Applications:
- Monitor the levels of reducing sugars, thereby helping in the detection of chitinases
- Used for determining protein concentration
CAS Number:Â Â Â Â Â Â 979-88-4;
Synonyms:Â Â Â Â Â Â Â Â Â Sodium [2,2′-biquinoline]-4,4′-dicarboxylate; 2,2′-Biquinoline-4,4-dicarboxylic acid disodium salt; [2,2′-Biquinoline]-4,4′-dicarboxylic acid, disodium salt; Disodium 2,2′-biquinoline-4,4′-dicarboxylate; 2,2′-Biquinoline-4,4′-dicarboxylic acid disodium salt
Molecular Formula: C20H10N2Na2O4
Molecular Weight:Â Â 388.29 g/mol (anhydrous basis)
InChI Key:Â Â Â Â Â Â Â Â Â Â AUPXFICLXPLHBB-UHFFFAOYSA-L
