Description
General Description
Biotin-PEG3-Amine is a water-soluble biotin compounds containing a polyethylene glycol (PEG) spacer arm and a terminal primary amine for conjugation via EDC and other crosslinking procedures. The short PEG segment in this compound is hydrophilic and confer greater solubility to labeled proteins. The primary amines of these pegylated biotin reagents can be conjugated to carboxyl groups on carboxy terminal, aspartate or glutamate residues using EDC, DCC or activated esters e.g. NHS esters. EDC activates carboxyl groups to bind to the -NH2 group of the
amino-biotin, forming an amide bond.
Chemical Name: (+)-Biotinyl-3,6,9-trioxa dioxaundecaneediamine
Molecular Formula: C18H34N4O5S
Molecular Weight: 418.55
CAS NO.: 359860-27-8
Purity: 98%
Spacer arm length: 22.9A0
Form: White to off-white solid
Solubility: ~25mg/ml in water
Storage: 40C
Features of Biotin-PEG3-Amine:
• Biotinylation— label molecules and surfaces for assays or affinity purification methods involving avidin or streptavidin probes and resins
• Amine-activated—primary amine can be crosslinked to proteins and material surfaces using EDC and other crosslinkers
• Pegylated—polyethylene glycol (PEG) groups in spacer arm enhances water solubility of biotinylated molecules
• Medium length—spacer arm (total length added to target) is 22.9 Å
Additional information
Size | 50 mg |
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