Description
Aliases
Bontoxilysin-A, BOTOX, atx, bna, botA
Antibody Type
Polyclonal Antibody
Species
Clostridium botulinum
Uniprot ID
P10845
Immunogen
Recombinant Clostridium botulinum Botulinum neurotoxin type A protein (1-436AA)
Raised In
Rabbit
Species Reactivity
Clostridium botulinum
Tested Applications
ELISA;Not yet tested in other applications.
Background / Function
Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure.
Isotype
IgG
Conjugate
Unconjugated
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Modification
BoNT/A
Literature
[1]”Crystal structure of botulinum neurotoxin type A and implications for toxicity.” Lacy D.B., Tepp W., Cohen A.C., Dasgupta B.R., Stevens R.C. Nat. Struct. Biol. 5:898-902(1998). [2]”SV2 is the protein receptor for botulinum neurotoxin A.” Dong M., Yeh F., Tepp W.H., Dean C., Johnson E.A., Janz R., Chapman E.R. Science 312:592-596(2006). [3]”Site-directed mutagenesis identifies active-site residues of the light chain of botulinum neurotoxin type a.” Rigoni M., Caccin P., Johnson E.A., Montecucco C., Rossetto O. Biochem. Biophys. Res. Commun. 288:1231-1237(2001).Additional information
| Size | 50μg, 100μg |
|---|
