General description
Bovine serum albumin (BSA) is a 67 kDa protein with three domains, each consisting of two subdomains. It is α-helical globular and a non-glycosylated protein. BSA is an established transporter for drugs, hormones and fatty acids. It is commonly used blocking agent in enzyme linked immunosorbent assay (ELISA) for preventing non-specific binding of antigens and antibodies to the microtiter plates. The cysteine and tyrosine residues of BSA have high affinity for metal binding.
Biological source:Â Â Â Â Â Â Bovine
Form:Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Lyophilized powder
Quality:Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Suitable for RIA
Molecular Weight:Â Â Â Â Â Â Single polypeptide protein (67kDa)
CAS No.:Â Â Â Â Â Â Â Â Â Â Â Â Â Â 9048-46-8
pH:Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â 5.2
EC Number:Â Â Â Â Â Â Â Â Â Â Â Â MFCD00130384 59 lysine residues; 30-35 are capable of conjugating
Solubility:Â Â Â Â Â Â Â Â Â Â Â Â Â Â Water (40 mg/ml)
Storage temp.:         2-8°C
Applications:
- as a blocking agent in enzyme linked immunosorbent assay (ELISA)
- as a component of Dulbecco PBS medium for preantral follicles culture
- as a component of modified Tris-buffered medium (mTBM) for oocyte insemination
