Aliases
Coat protein VP1, Coat protein VP2
Antibody Type
Polyclonal Antibody
Species
Human parvovirus B19
Uniprot ID
P07299
Immunogen
Recombinant Human parvovirus B19 Capsid protein VP2 protein (228-781AA)
Raised In
Rabbit
Species Reactivity
Human parvovirus B19
Tested Applications
ELISA;Not yet tested in other applications.
Background / Function
Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of two size variants of the capsid proteins, VP1 and VP2, which differ by the presence of an N-terminal extension in the minor protein VP1. The capsid encapsulates the genomic ssDNA. Capsid proteins are responsible for the attachment to host cell receptors, such as the glycosphingolipid globoside or the integrin heterodimer ITGAV/ITGB1. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptors also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell. Intracytoplasmic transport involves microtubules and interaction between capsid proteins and host dynein. Exposure of nuclear localization signal probably allows nuclear import of capsids (By similarity).
Isotype
IgG
Conjugate
Unconjugated
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Modification
Capsid protein VP2
Literature
[1]”Advances in human B19 erythrovirus biology.” Servant-Delmas A., Lefrere J.J., Morinet F., Pillet S. J. Virol. 84:9658-9665(2010). [2]”The globoside receptor triggers structural changes in the B19 virus capsid that facilitate virus internalization.” Boensch C., Zuercher C., Lieby P., Kempf C., Ros C. J. Virol. 84:11737-11746(2010). [3]”Alpha5beta1 integrin as a cellular coreceptor for human parvovirus B19: requirement of functional activation of beta1 integrin for viral entry.” Weigel-Kelley K.A., Yoder M.C., Srivastava A. Blood 102:3927-3933(2003).
