Caspase-12 Substrate (Ac-ATAD-AFC) [1mM], Fluorometric
General description
This product is a ready-to-use fluorometric substrate for caspases that recognize the amino acid sequence ATAD. Caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate ATAD-AFC at Ex/Em = 400/505 nm using a fluorometer or a fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.
Synonyms: Ac-ATAD-AFC, Caspase-12 Substrate, Fluorogenic
Peptide Sequence: Ac-Ala-Thr-Ala-Asp-AFC
Appearance: Liquid
Formulation: DMSO (4 mM)
Molecular Formula: C₂₆H₃₀F₃N₅O₁₀
Molecular Weight: 629.54
Handling: Protect from light and moisture
Storage temp.: -20°C