Description
Caspase-8 Substrate (Ac-IETD-AFC) [1mM], Fluorometric
General description
This product is a ready-to-use fluorometric substrate for FLICE/Caspase-8 and related caspases that recognize the amino acid sequence IETD. The sequence IETD is based on Caspase-8 cleavage site in CPP32/Caspase-3 proenzyme. FLICE and related caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate IETD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.
Synonyms: Ac-IETD-AFC, Caspase-8 Substrate, Fluorogenic
Peptide Sequence: Ac-Ile-Glu-Thr-Asp-AFC
Appearance: Liquid
Formulation: DMSO (2 mM)
Molecular Formula: C₃₁H₃₈F₃N₅O₁₂
Molecular Weight: 729.7
InChi Key: CGUOWXRXLQXOKO-FCQBACSVSA-N
PubChem CID: 25108570
Handling: Protect from light and moisture
Storage temp.: -20°C
Additional information
| Size | 250µl |
|---|
