Aliases
ICE-like apoptotic protease 5
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q14790
NCBI Protein: NP_001219.2
Immunogen
Peptide sequence around aa.217~221 (S-E-S-Q-T) derived from Caspase 8.
Raised In
Rabbit
Species Reactivity
Human
Tested Applications
WB Recommended dilution: Predicted MW: 18, 43, 57kd, Western blotting: 1:500~1:1000,
Background / Function
Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.Himeji D.et.al.(2002)Blood 99:4070-4078Breckenridge D.G.et.al. (2002)Proc. Natl. Acad. Sci. U.S.A. 99:4331-4336Muzio M.et.al. (1997)J. Biol. Chem. 272:2952-2956
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
