Description
Aliases
C/EBP-homologous protein; DDIT3; GADD153; DNA-damage inducible transcript 3;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot#: P35638;
NCBI Gene#: 1649;
N
Immunogen
Peptide sequence around phosphorylation site of serine30(V-L-S(p)-S-D) derived from Human CHOP .
Raised In
Rabbit
Species Reactivity
Human Mouse
Tested Applications
WB Recommended dilution: Western blotting: 1:500~1:1000
Background / Function
Multifunctional transcription factor in ER stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L. Ubeda M, Habener JF (2003) J Biol Chem 278, 40514-20
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser30
Additional information
| Size | 100?l |
|---|
