Aliases
MHC class II transactivator; CIITA; CIITA; MHC2TA;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P33076
NCBI Gene ID: 4261
Immunogen
Synthesized peptide derived from internal of human CIITA.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB Recommended dilution: Western blotting: 1:500~1:3000
Background / Function
Essential for transcriptional activity of the HLA class II promoter; activation is via the proximal promoter. No DNA binding of in vitro translated CIITA was detected. May act in a coactivator-like fashion through protein-protein interactions by contacting factors binding to the proximal MHC class II promoter, to elements of the transcription machinery, or both. Alternatively it may activate HLA class II transcription by modifying proteins that bind to the MHC class II promoter. Also mediates enhanced MHC class I transcription; the promoter element requirements for CIITA-mediated transcription are distinct from those of constitutive MHC class I transcription, and CIITA can functionally replace TAF1 at these genes. Exhibits intrinsic GTP-stimulated acetyltransferase activity. Exhibits serine/threonine protein kinase activity: can phosphorylate the TFIID component TAF7, the RAP74 subunit of the general transcription factor TFIIF, histone H2B at ‘Ser-37’ and other histones (in vitro).Steimle V., Cell 75:135-146(1993).Al-Kandari W., J. Mol. Biol. 369:1175-1187(2007).Al-Kandari W., Mol. Immunol. 44:311-321(2007).Croager E.J., Biochim. Biophys. Acta 1353:231-235(1997).
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
