Aliases
CKII; CK2A1; CSNK2A1
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P68400
NCBI Protein: NP_001894.2
Immunogen
Peptide sequence around phosphorylation site of threonine360/serine 362 (V-P-T(p)-P-S(p)-P-L) derived from Human CK2a.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB Recommended dilution: Predicted MW: 42kd, Western blotting: 1:1000,
Background / Function
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The a and a’ chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates ‘Ser-392’ of p53/TP53 following UV irradiation.Keller D.M., Zeng X., et al., Mol. Cell 7:283-292(2001) Keller D.M., Lu H. et al., J. Biol. Chem. 277:50206-50213(2002)Trembley J.H., Tatsumi S., et al., Mol. Cell. Biol. 25:1446-1457(2005)Niefind K., Guerra B., et al., Acta Crystallogr. D 56:1680-1684(2000)
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Thr360/Ser362
