General Description
Coelenterazine 400 a is a coelenterazine derivative that serves as a substrate for Renilla luciferase (Rluc) and generates an emission peak centered around 400 nm. It does not oxidize well with Gaussia (GLuc). It is the preferred substrate for BRET (Bioluminescence Resonance Energy Transfer) since its emission maximum of 400nm has minimal interference with the GFP emission.
CAS Number:Â Â Â Â Â Â Â Â Â 70217-82-2;
Synonyms:Â Â Â Â Â Â Â Â Â Â Â BlueSyn C; DeepBlueC; C-7011; 2,8-dibenzyl-6-phenyl-7H-imidazo[1,2-a]pyrazin-3-one;
Molecular Formula:Â Â Â C26H21N3O;
Molecular Weight:Â Â Â Â 391.474 g/mol
InChI Key:Â Â Â Â Â Â Â Â Â Â Â Â MWOYPGFRXPPBOX-UHFFFAOYSA-N