Aliases
Cullin-2; CUL-2;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q13617
NCBI Gene ID: 8453
Immunogen
Synthesized peptide derived from human Cullin 2.
Raised In
Rabbit
Species Reactivity
Human Mouse
Tested Applications
WB IHC IF Recommended dilution: Western blotting: 1:500~1:3000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:500
Background / Function
Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 By similarity. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).Seiji Shiraishi, J. Biol. Chem., Mar 2007; 282: 9017 – 9028.Isabelle Groulx, J. Biol. Chem., Mar 2000; 275: 8991.Dimitris Liakopoulos, PNAS, May 1999; 96: 5510.Kyungwon Huh, AACR Meeting Abstracts, Apr 2006; 2006: 1326.
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
