Aliases
Cytochrome P450 3A4; EC 1.14.13.67; Quinine 3-monooxygenase; CYPIIIA4; Nifedipine oxidase
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P08684
NCBI Gene ID: 1576
Immunogen
Synthesized peptide derived from internal of human Cytochrome P450 3A4.
Raised In
Rabbit
Species Reactivity
Human Mouse
Tested Applications
WB IHC Recommended dilution: Western blotting: 1:500~1:3000, Immunohistochemistry: 1:50~1:100,
Background / Function
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1′-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,8-cineole 2-exo-monooxygenase. The enzyme also hydroxylates etoposide.Vuoristo M.M., J. Biol. Chem. 270:22873-22881(1995).Mungall A.J., Nature 425:805-811(2003).Lui V.C., Genomics 32:401-412(1996).
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
