Aliases
E2F-4; p107/p130-binding protein;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q16254
NCBI Gene ID: 1874/1875
Immunogen
Synthesized peptide derived from human E2F4.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC Recommended dilution: Western blotting: 1:500~1:3000, Immunohistochemistry: 1:50~1:100,
Background / Function
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5′-TTTC[CG]CGC-3′ found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1.Kathryn M. Kinross, Blood, Aug 2006; 108: 886 – 895.Dawei Wang, Mol. Cell. Biol., May 2000; 20: 3417 – 3424.Stephan P. Persengiev, Mol. Cell. Biol., Sep 1999; 19: 6048 – 6056.Stefan Gaubatz, Mol. Cell. Biol., Feb 2001; 21: 1384 – 1392.
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
