Aliases
hEED, WD protein associating with integrin cytoplasmic tails 1, WAIT-1, EED
Antibody Type
Polyclonal Antibody
Species
Human
Uniprot ID
O75530
Immunogen
Recombinant human Polycomb protein EED protein (1-300AA)
Raised In
Rabbit
Species Reactivity
Human
Tested Applications
ELISA, WB, IHC ;Recommended dilution:WB:1:500-2000, IHC:1:20-1:200
Background / Function
Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates ‘Lys-9’ and ‘Lys-27’ of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes ‘Lys-26’ trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 ‘Lys-27’, whereas H3 ‘Lys-27’ recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
Isotype
IgG
Conjugate
Unconjugated
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form
Liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antigen Affinity Purified
Literature
[1]Binding of different histone marks differentially regulates the activity and specificity of polycomb repressive complex 2 (PRC2).”Xu C., Bian C., Yang W., Galka M., Ouyang H., Chen C., Qiu W., Liu H., Jones A.E., MacKenzie F., Pan P., Li S.S., Wang H., Min J.Proc. Natl. Acad. Sci. U.S.A. 107:19266-19271(2010). [2]System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.”Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.Sci. Signal. 4:RS3-RS3(2011). [3]Corepressor protein CDYL functions as a molecular bridge between polycomb repressor complex 2 and repressive chromatin mark trimethylated histone lysine 27.” Zhang Y., Yang X., Gui B., Xie G., Zhang D., Shang Y., Liang J.J. Biol. Chem. 286:42414-42425(2011).
