Aliases
mRNA cap-binding protein; eIF-4F 25 kDa subunit;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P06730
NCBI Protein: NP_001124150.
Immunogen
Peptide sequence around phosphorylation site of serine 209 (S-G-S(p)-T-T) derived from Human eIF4E.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC IF Recommended dilution: Predicted MW: 25kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background / Function
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Li BD, et al. (1998) Ann Surg; 227(5): 756-763 Altmann M, et al. (1989) Nucleic Acids Res; 17(18): 7520 De Gregorio E, et al. (2001) RNA; 7(1): 106-113 Gu W, et al. (2004) Nucleic Acids Res; 32(15): 4448-4461
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser209
