Description
Aliases
ENX-1, Enhancer of zeste homolog 2, Lysine N-methyltransferase 6, EZH2, KMT6
Antibody Type
Polyclonal Antibody
Species
Human
Uniprot ID
Q15910
Immunogen
Recombinant human Histone-lysine N-methyltransferase EZH2 protein (1-250AA)
Raised In
Rabbit
Species Reactivity
Human,Mouse
Tested Applications
ELISA, WB;Recommended dilution:WB:1:500-5000
Background / Function
Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates ‘Lys-9’ (H3K9me) and ‘Lys-27’ (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate ‘Lys-27’ of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer; involved in the di and trimethylation of ‘Lys-27’ of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.
Isotype
IgG
Conjugate
Unconjugated
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form
Liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antigen Affinity Purified
Literature
[1]Mutations in EZH2 cause Weaver syndrome.” Gibson W.T., Hood R.L., Zhan S.H., Bulman D.E., Fejes A.P., Moore R., Mungall A.J., Eydoux P., Babul-Hirji R., An J., Marra M.A., Chitayat D., Boycott K.M., Weaver D.D., Jones S.J.Am. J. Hum. Genet. 90:110-118(2012). [2]Mutational spectrum analysis of chronic myelomonocytic leukemia includes genes associated with epigenetic regulation: UTX, EZH2, and DNMT3A.” Jankowska A.M., Makishima H., Tiu R.V., Szpurka H., Huang Y., Traina F., Visconte V., Sugimoto Y., Prince C., O’Keefe C., Hsi E.D., List A., Sekeres M.A., Rao A., McDevitt M.A., Maciejewski J.P.Blood 118:3932-3941(2011). [3]Somatic mutations altering EZH2 (Tyr641) in follicular and diffuse large B-cell lymphomas of germinal-center origin.”Morin R.D., Johnson N.A., Severson T.M., Mungall A.J., An J., Goya R., Paul J.E., Boyle M., Woolcock B.W., Kuchenbauer F., Yap D., Humphries R.K., Griffith O.L., Shah S., Zhu H., Kimbara M., Shashkin P., Charlot J.F. Marra M.A.Nat. Genet. 42:181-185(2010).Additional information
| Size | 50μl, 100μl |
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