Aliases
Gamma-aminobutyric acid receptor subunit alpha-3; Gabra3;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P20236
NCBI Protein: NP_058765.1
Immunogen
Peptide sequence around aa. 33~37(R-R-Q-E-P)derived from Rat GABA A Receptor a3.
Raised In
Rabbit
Species Reactivity
Human Rat Mouse
Tested Applications
WB Recommended dilution: Predicted MW: 51kd, Western blotting: 1:500~1:1000,
Background / Function
GABA (g-aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system and interacts with three different receptors: GABA(A), GABA(B) and GABA(C) receptor. The ionotropic GABA(A) and GABA(C) receptors are ligand-gated ion channels that produce fast inhibitory synaptic transmission. In contrast, the metabotropic GABA(B) receptor is coupled to G proteins that modulate slow inhibitory synaptic transmission (1). Functional GABA(B) receptors form heterodimers of GABA(B)R1 and GABA(B)R2 where GABA(B)R1 binds the ligand and GABA(B)R2 is the primary G protein contact site (2). Two isoforms of GABA(B)R1 have been cloned: GABA(B)R1a is a 130 kD protein and GABA(B)R1b is a 95 kD protein (3). G proteins subsequently inhibit adenyl cylase activity and modulate inositol phospholipid hydrolysis. GABA(B) receptors have both pre- and postsynaptic inhibitions: presynaptic GABA(B) receptors inhibit neurotransmitter release through suppression of high threshold calcium channels, while postsynaptic GABA(B) receptors inhibit through coupled activation of inwardly rectifying potassium channels. In addition to synaptic inhibition, GABA(B) receptors may also be involved in hippocampal long-term potentiation, slow wave sleep and muscle relaxation (1).Jones, K.A. et al. (2000) Neuropsychopharmacology 23, S41-9. Duthey, B. et al. (2002) J Biol Chem 277, 3236-41. Kaupmann, K. et al. (1997) Nature 386, 239-46.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide
