Genomic DNA Extraction Kit – Blood

Description

Our Genomic DNA Extraction – Blood (GDEB) kit is designed for the isolation of genomic DNA from whole blood, bone marrow and buffy coat. The DNA extraction kit uses the principle of cell lysis, protein precipitation and finally DNA precipitation to isolate high quality genomic DNA from whole blood. A simple and easy to follow step process is all that’s needed, taking between 30-60 minutes with five major steps- RBC Lysis, Cell lysis, Protein removal, Harvest DNA pellet, and DNA Hydration. RBC Lysis Buffer liquefies the cell membrane and removes RBC.The DNA Extraction Kit – Blood protocol has been designed to use 0.5 mL whole blood; however, the protocol can be easily adapted for larger sample volumes. The purified DNA will have an A₂₆₀/A₂₈₀ ratio between 1.8 ‐2.0 and yields ranging between 10µg to 15µg/mL depending on volume of blood. The extracted DNA is suitable for PCR, southern blotting, and other downstream applications.

Additional information