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Our Genomic DNA Extraction Kit – Gram(+) Bacteria / Yeast / Fungi kit is specially designed for extracting genomic DNA from Gram positive bacteria, Yeast, and Fungi. Optimized chaotropic salt and detergents are used to extract genomic DNA efficiently by pre-lysis and lysis buffer system. The kit provides a fast and easy way to purify DNA from various Gram-positive bacteria, yeast, fungal tissue and fungi. The method involves preparation of spheroplast, using the enzymes: lysozyme (in bacteria & fungi) or lyticase (in yeast) and the DNA present in the sample is then selectively bound to the spin column provided with the kit. After washing the spin column with the Wash Buffer, the DNA is eluted from the column with a small volume of an elution buffer, TE. The isolated DNA is suitable for PCR and other downstream applications. The supplied kit is suitable for 50 sample preparations.
Kit Components:
| Items | Cat. No. 10645-0
(50 Preps) |
Cat. No. 10645-1
(100 Preps) |
Storage |
| Suspension Buffer | 15 ml | 30 ml | 4°C |
| Lysis Buffer | 25 ml | 50 ml | 4°C |
| Binding Buffer | 15 ml | 30 ml | 4°C |
| Wash Buffer (Concentrate) | 10 ml | 20 ml | 4°C |
| Lysozyme | 0.2 ml | 0.4 ml | -20°C |
| Lyticase | 0.2 ml | 0.4 ml | -20°C |
| RNase A | 0.3 ml | 0.6 ml | -20°C |
| Proteinase K | 0.5 ml | 1.0 ml | -20°C |
| Elution Buffer | 5 ml | 10 ml | 4°C |
| Spin Column with Collection Tube | 50 Columns | 100 Columns | RT |
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