Description
General Description
GMBS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a short spacer arm of 7.3 Å in length. It has a low potential for eliciting an immune response compared to SMCC, thus ensuring the primary response is to the antigen-carrier protein conjugate and not against a determinant on the crosslinking reagent.
CAS No.: 80307-12-6
Molecular Weight: 280.23
Molecular Formula: C12H12N2O6
Spacer Arm: 7.3 Å
Features of GMBS:
- Reactive groups: NHS ester and maleimide
- Reactive toward: amino and sulfhydryl groups
- C4-homolog of aliphatic spacer series which includes AMAS, BMPS, GMBS and EMCS
- NHS ester end couples with primary amines at pH 7-9 to form stable amide bonds
- Maleimides react with -SH groups at pH 6.5-7.5, forming stable thioether linkages
- Non-cleavable
- Water-insoluble (dissolve first in DMF or DMSO); compare to Sulfo-GMBS
- Short aliphatic spacer has low potential for eliciting an immune response
Additional information
| Size | 100 mg |
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