Antibodies - Cepham
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Antibodies - Cepham
Goat a-Rabbit IgG, Alkaline phosphatase (AP) Labeled (1mg/ml) $313.95
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Goat a-Mouse IgG, Horseradish peroxidase (HRP) labeled (1mg/ml)

$310.80

Size

1.0ml
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SKU: 10025 Categories: ,
Description

Product name: Goat Anti-Mouse IgG H&L (HRP)

Host species: Goat

Target species: Mouse

Specificity: By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.

Tested applications: Suitable for: Western Blot, ELISA, ICC, IHC-P

Conjugation: HRP

Form: Liquid

Storage instructions: Shipped at 4°C. Store at +4°C

Storage buffer: 0.2% BSA, PBS

Preservative: 0.1% Proclin

Concentration: 1 mg/ml

Purity: Immunogen affinity purified: isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

Conjugation notes: Molar enzyme/ antibody protein ratio is 4:1

Clonality: Polyclonal

Isotype: IgG

Recommended Dilutions: Below are the recommended starting dilutions; optimal dilutions/concentrations and should be determined by the end user:

Application

Recommended Dilution

Western Blot

1:200 – 1:1000
Chemiluminescent detection

1:1000 – 1:5000
Colorimetric detection

ELISA

1:50000 – 1:100000


Fig: Western blot – Probed with Goat Anti-Mouse IgG H&L (HRP) 
All lanes: Anti-beta Actin antibody, Loading Control at 1 µg/ml
Lanes 1-6: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate 
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1 – 2: Goat α-Mouse IgG, Horseradish peroxidase (HRP) labeled at 1:2000 dilution
Lanes 3 – 4: Goat α-Mouse IgG, Horseradish peroxidase (HRP) labeled at 1:10000 dilution
Lanes 5 – 6: Goat α-Mouse IgG, Horseradish peroxidase (HRP) labeled at 1:20000 dilution
Developed using the ECL technique
Exposure time: 10 seconds

Manual
Download Manual
Safety Datasheet
Download Safety Datasheet
Manual
Certificate of Analysis
Download Certificate of Analysis
Safety Datasheet

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Certificate of Analysis

Certificate of Analysis

Shipping & Delivery
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