Product name: Goat α-Rabbit IgG, Horseradish peroxidase (HRP) labeled
Host species: Goat
Target species: Rabbit
Immunogen: Rabbit IgG, whole molecule
Conjugation: HRP
Form: Liquid
Constituents: 1% BSA, 30% Glycerol, PBS, pH 7.4
Preservative: 0.1% Proclin
Concentration: 1 mg/ml
Purity: Immunogen affinity purified
Purification notes: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.
Conjugation notes: Horseradish Peroxidase (HRP)
Clonality: Polyclonal
Isotype: IgG
Tested applications: Western Blot, Dot blot, ELISA, IHC-P, ICC, IHC
Storage instructions: Shipped at 4°C. Store at +4°C
Recommended Dilutions: Below are the recommended starting dilutions; optimal dilutions/concentrations and should be determined by the end user:
Application |
Recommended Dilution |
Western Blot |
1:2000 – 1:20000 Chemiluminescent |
Fig- Western Blot: Probed with Goat α-Rabbit IgG, Horseradish peroxidase (HRP) labeled
All lanes: Anti-NEK7 antibody at 1/2000 dilution
All lanes: Mouse brain tissue cytoplasmic lysate- Proteins at 10 µg per lane
Secondary All lanes: Goat Anti-Rabbit IgG H&L (HRP) at 1:5000 dilution
Developed using the ECL technique
Exposure time: 10 seconds