Description
Product name: Goat anti-Rabbit IgG, Horseradish peroxidase HRP-labeled
Host species: Goat
Target species: Rabbit
Immunogen: Rabbit IgG, whole molecule
Conjugation: HRP
Form: Liquid
Constituents: 0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6
Concentration: Varies from batch to batch, generally 0.1mg/mL
Purity: Immunogen affinity purified
Purification notes: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Conjugation notes: Horseradish Peroxidase (HRP)
Clonality: Polyclonal
Isotype: IgG
Tested applications: Western Blot, Dot blot, ELISA, IHC-P, ICC, IHC
Storage instructions: Shipped at 4°C. Upon receipt, store at -20°C or -80°C. Avoid repeated freeze and thaw.
Recommended Dilutions: Below are the recommended starting dilutions; optimal dilutions/concentrations and should be determined by the end user:
|
Application |
Recommended Dilution |
|
Western Blot ELISA IHC ICC |
1:500 – 1:5000 1:500-1:10000 1:50-1:500 1:50-1:500 |
Fig- Western Blot: Probed with Goat α-Rabbit IgG, Horseradish peroxidase (HRP) labeled
All lanes: Anti-NEK7 antibody at 1/2000 dilution
All lanes: Mouse brain tissue cytoplasmic lysate- Proteins at 10 µg per lane
Secondary All lanes: Goat Anti-Rabbit IgG H&L (HRP) at 1:5000 dilution
Developed using the ECL technique
Exposure time: 10 seconds
Additional information
| Size | 0.5 mL, 1.0 mL |
|---|
