Goat anti-Rabbit IgG, Horseradish peroxidase-HRP-labeled

$152.25$288.75

Goat Anti-Rabbit IgG (H+L) Antibody; HRP conjugated, purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Description

Product name: Goat anti-Rabbit IgG, Horseradish peroxidase HRP-labeled

Host species: Goat

Target species: Rabbit

Immunogen: Rabbit IgG, whole molecule

Conjugation: HRP

Form: Liquid

Constituents: 0.01M Sodium phosphate, 0.25M NaCl, 50% glycerol, pH7.6

Concentration: Varies from batch to batch, generally 0.1mg/mL

Purity: Immunogen affinity purified

Purification notes: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Conjugation notes: Horseradish Peroxidase (HRP)

Clonality: Polyclonal

Isotype: IgG

Tested applications: Western Blot, Dot blot, ELISA, IHC-P, ICC, IHC

Storage instructions: Shipped at 4°C. Upon receipt, store at -20°C or -80°C. Avoid repeated freeze and thaw.

Recommended Dilutions: Below are the recommended starting dilutions; optimal dilutions/concentrations and should be determined by the end user:

Application

Recommended Dilution

Western Blot

ELISA

IHC

ICC

1:500 – 1:5000

1:500-1:10000

1:50-1:500

1:50-1:500

Fig- Western Blot: Probed with Goat α-Rabbit IgG, Horseradish peroxidase (HRP) labeled

All lanes: Anti-NEK7 antibody at 1/2000 dilution

All lanes: Mouse brain tissue cytoplasmic lysate- Proteins at 10 µg per lane

Secondary All lanes: Goat Anti-Rabbit IgG H&L (HRP) at 1:5000 dilution

 

Developed using the ECL technique

Exposure time: 10 seconds

Additional information

Size

0.5 mL, 1.0 mL

Certificate of Analysis