Aliases
H2BQ; GL105; H2B.1; H2BFQ; H2BGL105
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P33778
NCBI Protein: NP_003519.1
Immunogen
Peptide sequence around aa.41~45 (Y-V-Y-K-V) derived from Human H2B.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB Recommended dilution: Predicted MW: 14kd, Western blotting: 1:500~1:1000,
Background / Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.Borchert GM,et al. (2010) PLoS One.5(7):e11641.Pennini ME,et al.(2010) PLoS Pathog.6(7):e1000995.Lu C,et al. (2010) Sci China Life Sci.53(6):663-8.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
