Aliases
Tyrosine kinase-type cell surface receptor HER3
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P21860
NCBI Protein: NP_001005915.
Immunogen
Peptide sequence around phosphorylation site of tyrosine 1328 (P-D-Y(p)-W-H) derived from Human Her3/ErbB3.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC IF Recommended dilution: Predicted MW: 185kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background / Function
Binds and is activated by neuregulins and NTAK.Holbro, T. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 8933-8938.Kobayashi, M. et al. (2003) Oncogene 22, 1294-1301. Kim, H.H. et al. (1994) J. Biol. Chem. 269, 24747-24755.Yarden, Y. and Sliwkowski, M.X. (2001) Nature Rev. Mol. Cell. Biol. 2, 127-137.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Tyr1328
