Description
Aliases
HSF 1; HSTF1; Heat shock factor protein 1;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot#: Q00613;
NCBI Gene#: 3297;
N
Immunogen
Peptide sequence around phosphorylation site of threonine 142(L-L-T(p)-D-V) derived from Human HSF1 .
Raised In
Rabbit
Species Reactivity
Human Mouse
Tested Applications
IHC Recommended dilution: Immunohistochemistry: 1:50~1:100
Background / Function
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. Rabindran S.K., Proc. Natl. Acad. Sci. U.S.A. 88:6906-6910(1991).The MGC Project Team; Genome Res. 14:2121-2127(2004).Schuetz T.J.,Proc. Natl. Acad. Sci. U.S.A. 88:6911-6915(1991)
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Thr142
Additional information
| Size | 100?l |
|---|
