Description
Aliases
HSTF 1
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q00613
NCBI Protein: NP_005517.1
Immunogen
Peptide sequence around phosphorylation site of serine 303 (P-P-S(p)-P-P) derived from Human HSF1.
Raised In
Rabbit
Species Reactivity
Human
Tested Applications
WB IHC Recommended dilution: Predicted MW: 82kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background / Function
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. Parvaneh Rafiee, et,al. (2006) Am J Physiol Cell Physiol ; 291: C931 – C945 Fumika Shinozaki, et,al. (2006) J. Biol. Chem ; 281: 16361 – 16369. Eiichi Takaki, et,al. (2006) J. Biol. Chem ; 281: 4931 – 4937. Jan-Jong Hung, et,al. (1998) J. Biol. Chem ; 273: 31924.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser303
Additional information
| Size | 100?l |
|---|
