Aliases
59 kDa serine/threonine protein kinase; EC 2.7.11.1; ILK-1; ILK1; Integrin-linked protein kinase
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q13418
NCBI Gene ID: 3611
Immunogen
Synthesized non-phosphopeptide derived from human ILK around the phosphorylation site of serine 246 (I-F-S(p)-H-P).
Raised In
Rabbit
Species Reactivity
Human Mouse
Tested Applications
WB IHC Recommended dilution: Western blotting: 1:500~1:3000, Immunohistochemistry: 1:50~1:100,
Background / Function
Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.Hannigan G.E.,Nature 379:91-96(1996).Janji B.,Oncogene 19:3069-3077(2000).Tadic B.,Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
