Aliases
Polydeoxyribonucleotide synthase [NAD(+)], ligA, dnaL, lig, lop, pdeC, b2411, JW2403
Antibody Type
Polyclonal Antibody
Species
Escherichia coli
Uniprot ID
P15042
Immunogen
Recombinant Escherichia coli DNA ligase protein (1-671AA)
Raised In
Rabbit
Species Reactivity
Escherichia coli
Tested Applications
ELISA, WB;Not yet tested in other applications.
Background / Function
DNA ligase that catalyzes the formation of phosphodiester linkages between 5′-phosphoryl and 3′-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.
Isotype
IgG
Conjugate
Unconjugated
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Research
Others
Literature
[1] Nucleotide sequence of the lig gene and primary structure of DNA ligase of Escherichia coli. Ishino Y., Shinagawa H., Makino K., Tsunasawa S., Sakiyama F., Nakata A.Mol. Gen. Genet. 204:1-7(1986) [2] Ally A., Ally D., Zhang X., Robichaud M., Backma
