Aliases
CSDD2; FLJ16517;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q6ZN17
NCBI Protein: NP_001004317.
Immunogen
Peptide sequence around aa.242~246( P-S-V-Q-K) derived from Human LIN28B
Raised In
Rabbit
Species Reactivity
Human
Tested Applications
WB Recommended dilution: Predicted MW: 21 32kd, Western blotting: 1:500~1:1000,
Background / Function
Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5′-GGAG-3′ motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5′-GGAG-3′ motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth. Identification and characterization of lin-28 homolog B (LIN28B) in human hepatocellular carcinoma.Guo Y., Chen Y., Ito H., et al.Gene 384:51-61(2006) Lin28 mediates the terminal uridylation of let-7 precursor MicroRNA.Heo I., Joo C., Cho J., Ha M., Han J., Kim V.N. Mol. Cell 32:276-284(2008) TUT4 in concert with Lin28 suppresses MicroRNA biogenesis through pre-microRNA uridylation.Heo I., Joo C., Kim Y.-K., Kim V.N.,et al. Cell 138:696-708(2009)
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
