Aliases
100 kDa thyroid hormone receptor associated protein; ARC100; Activator-recruited cofactor 100 kDa component; DRIP100; KIAA0130
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: O75448
NCBI Gene ID: 9862
Immunogen
Synthesized peptide derived from internal of human MED24.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IF Recommended dilution: Western blotting: 1:500~1:3000Immunofluorescence: 1:100~1:500
Background / Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.Yuan C.-X., Proc. Natl. Acad. Sci. U.S.A. 95:7939-7944(1998).Rachez C., Genes Dev. 12:1787-1800(1998).Nagase T., DNA Res. 2:167-174(1995).
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
