Aliases
ERK activator kinase 1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK 1
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q02750
NCBI Protein: NP_002746.1
Immunogen
Peptide sequence around aa. 289~293 (P-R-T-P-G) derived from Human MEK1.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC Recommended dilution: Predicted MW: 45kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100,
Background / Function
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.Kevin D. Burroughs, et,al. (2003) Mol. Cancer Res ; 1: 312. Michael J. Piatelli, et,al. (2002) J. Biol. Chem ; 277: 12144 – 12150. Margaret M. Morgan, et,al. (2001) J. Immunol ; 167: 5708. Herbert Schramek, et,al. (2003) Am J Physiol Cell Physiol, ; 285: C652 – C661.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
