Aliases
MGMT
Antibody Type
Polyclonal Antibody
Species
Human
Uniprot ID
P16455
Immunogen
Recombinant human Methylated-DNA–protein-cysteine methyltransferase protein (4-207AA)
Raised In
Rabbit
Species Reactivity
Human
Tested Applications
ELISA;Not yet tested in other applications.
Background / Function
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Isotype
IgG
Conjugate
HRP
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Modification
Methylated-DNA–protein-cysteine methyltransferase
Research
Epigenetics and Nuclear Signaling
Literature
[1] “Isolation and structural characterization of a cDNA clone encoding the human DNA repair protein for O6-alkylguanine.”Tano K., Shiota S., Collier J., Foote R.S., Mitra S.Proc. Natl. Acad. Sci. U.S.A. 87:686-690(1990) [2] “cDNA cloning and chromosom
