Aliases
mitochondrial 28S ribosomal protein S25; mitochondrial ribosomal protein S25; MRP-S25; RPMS25; RT25
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P82663
NCBI Gene ID: 64432
Immunogen
Synthesized peptide derived from internal of human MRPS25.
Raised In
Rabbit
Species Reactivity
Human Mouse
Tested Applications
WB Recommended dilution: Western blotting: 1:500~1:3000
Background / Function
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4.Ota T., Nat. Genet. 36-40-45(2004).Hattori A., DNA Res. 7:357-366(2000).Koc E.C., J. Biol. Chem. 275:32585-32591(2000).
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
