Description
Aliases
NP, Nucleocapsid protein, Protein N, N
Antibody Type
Polyclonal Antibody
Species
Rabies virus
Uniprot ID
P15197
Immunogen
Recombinant Rabies virus Nucleoprotein (1-450AA)
Raised In
Rabbit
Species Reactivity
Rabies virus
Tested Applications
ELISA;Not yet tested in other applications.
Background / Function
Encapsidates the genome in a ratio of one protein N per nine ribonucleotides, protecting it from nucleases. If expressed without protein P it binds non-specifically RNA and therefore can bind it’s own mRNA. Interaction with protein P abolishes any non-specific RNA binding, and prevents phosphorylation. The soluble N-P complex encapsidates specifically the genomic RNA, with protein N protecting the genome like a pearl necklace. The encapsidated genomic RNA is termed the nucleocapsid (NC) and serves as template for viral transcription and replication. Protein N binds protein P in the NC through a different interaction, and can be phosphorylated. Subsequent viral replication is dependent on intracellular concentration of newly synthesized protein N. During replication, encapsidation by protein N is coupled to RNA synthesis and all replicative products are resistant to nucleases
Isotype
IgG
Conjugate
FITC
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Research
Others
Literature
[1]”Sequence of the 3386 3′ nucleotides of the genome of the AVO1 strain rabies virus: structural similarities in the protein regions involved in transcription.” Poch O., Tordo N., Keith G.Biochimie 70:1019-1029(1988). [2]”CharacterizAdditional information
| Size | 50μg, 100μg |
|---|
