N-Hydroxysulfosuccinimide; Sulfo NHS

Description

N-hydroxysulfosuccinimide sodium salt (Sulfo NHS) is a water-soluble sulfonated analog of N-hydroxysuccinimide. It can undergo a coupling reaction with carboxylic acids in the presence of carbodiimides such as 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) to form hydrophilic N-hydroxysulfosuccinimide active esters that are useful in protein cross-linking studies. It enables control and modification of carbodiimide crosslinking reactions involving activation of carboxylates (—COOH) for conjugation with primary amines (—NH2). Derivatives are easily synthesized by mixing the Sulfo-NHS with a carboxyl-containing molecule and a dehydrating agent such as the carbodiimide EDC (EDAC). The method is the basis for generating multiple protein labeling reagents, including amine-reactive fluorescent dyes, biotin affinity tags and pegylation compounds.

SYNONYMS:                            Hydroxy-2,5-dioxo-pyrrolidine-3-sulfonic-acid sodium salt, and Sulfo-NHS

PURITY LIMIT:                       ≥ 99% (HPLC)

MOLECULAR FORMULA:     C₄H₄NNaO₆S

MOLECULAR WEIGHT:        217.13

CAS NO:                                 106627-54-7

MDL NO:                                MFCD00043100

APPEARANCE:                     Off-white to white powder

MELTING POINT:                 > 300 ºC

STORAGE TEMP:                 Store at 0 – 8 °C

Applications:

• improve efficiency of EDC coupling reactions
• convert carboxyls to amine-reactive Sulfo-NHS esters
• crosslink proteins to carboxyl-coated beads or surfaces more efficiently
• activate nanoparticles with amine-reactive Sulfo-NHS esters

Additional information