Aliases
Nuclear factor interleukin-3-regulated protein; E4 promoter binding-protein 4; Interleukin-3 promoter transcriptional activator; Transcriptional activator NF-IL3A; Interleukin-3 binding-protein 1
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q16649
NCBI Gene ID: 4783
Immunogen
Synthesized peptide derived from internal of human NFIL3.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB Recommended dilution: Western blotting: 1:500~1:3000
Background / Function
Acts as a transcriptional regulator that recognizes and binds to the sequence 5′-[GA]TTA[CT]GTAA[CT]-3′, a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts By similarity. Represses transcriptional activity of PER1 By similarity. Represses transcriptional activity of PER2 via the B-site on the promoter By similarity. Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF) By similarity. Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock By similarity. Protects pro-B cells from programmed cell death By similarity.Cowell I.G., Mol. Cell. Biol. 12:3070-3077(1992).Zhang W., Mol. Cell. Biol. 15:6055-6063(1995).Humphray S.J., Nature 429:369-374(2004).
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.