NHS-LC-Biotin

General Description

NHS-LC-Biotin is a long-chain, NHS-ester activated biotinylation reagent and is membrane permeable. It can be used for labeling primary amines (e.g., protein lysines) and used for general intracellular labeling, being membrane permeable.

Synonym:                         Succinimidyl 6-(biotinamido)hexanoate

CAS Number:                  72040-63-2

Molecular Weight:         454.55

Molecular Formula:       C₂₀H₃₀N₄O₆S

Spacer Arm:                    22.4 Å

Reactive group(s):         N-hydroxysuccinimide ester reacts with primary amines at pH 7.0-9.0

Features of NHS-LC-Biotin:

• Membrane-permeable— can be used to label inside cells (intracellular)

• Amine-reactive— reacts with primary amines (-NH2), such as the side-chain of lysines (K) or the amino-termini of polypeptides

• Protein labeling— biotinylate antibodies or other proteins for detection or purification using streptavidin probes or resins

• Irreversible— forms permanent amide bonds; spacer arm cannot be cleaved

• Solubility—must be dissolved in DMSO or DMF before further dilution in aqueous buffers

• Medium length— spacer arm 22.4 Å; and consists of the biotin valeric acid group extended by a 6-atom chain