Aliases
NMN adenylyltransferase 3, Nicotinate-nucleotide adenylyltransferase 3, NaMN adenylyltransferase 3, Pyridine nucleotide adenylyltransferase 3, PNAT-3FKSG76, NMNAT3
Antibody Type
Polyclonal Antibody
Species
Human
Uniprot ID
Q96T66
Immunogen
Recombinant human Nicotinamide mononucleotide adenylyltransferase 3 protein (1-215AA)
Raised In
Rabbit
Species Reactivity
Human,Mouse
Tested Applications
ELISA, WB, IHC;Recommended dilution:WB:1:200-1000, IHC:1:20-1:200
Background / Function
Catalyzes the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Can also use GTP and ITP as nucleotide donors. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD+. For the pyrophosphorolytic activity, can use NAD (+), NADH, NAAD, nicotinic acid adenine dinucleotide phosphate (NHD), nicotinamide guanine dinucleotide (NGD) as substrates. Fails to cleave phosphorylated dinucleotides NADP+, NADPH and NAADP+. Protects against axonal degeneration following injury.
Isotype
IgG
Conjugate
Unconjugated
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Modification
Nicotinamide mononucleotide adenylyltransferase 3 protein
Literature
[1]”Structural characterization of a human cytosolic NMN/NaMN adenylyltransferase and implication in human NAD biosynthesis.” Zhang X., Kurnasov O.V., Karthikeyan S., Grishin N.V., Osterman A.L., Zhang H. J. Biol. Chem. 278:13503-13511(2003) . [2]”Initial-rate kinetics of human NMN-adenylyltransferases: substrate and metal ion specificity, inhibition by products and multisubstrate analogues, and isozyme contributions to NAD+ biosynthesis.” Sorci L., Cimadamore F., Scotti S., Petrelli R., Cappellacci L., Franchetti P., Orsomando G., Magni G. Biochemistry 46:4912-4922(2007). [3]”Subcellular compartmentation and differential catalytic properties of the three human nicotinamide mononucleotide adenylyltransferase isoforms.” Berger F., Lau C., Dahlmann M., Ziegler M. J. Biol. Chem. 280:36334-36341(2005).
