Aliases
Methyltransferase , Putative papain-like cysteine protease, NTPase/helicase, RNA-directed RNA polymerase, RdRp, ORF1
Antibody Type
Polyclonal Antibody
Species
Hepatitis E virus genotype 1
Uniprot ID
P33424
Immunogen
Recombinant Hepatitis E virus genotype 1 Non-structural polyprotein pORF1 protein (60-240AA)
Raised In
Rabbit
Species Reactivity
Hepatitis E virus genotype 1
Tested Applications
ELISA;Not yet tested in other applications.
Background / Function
Methyltransferase displays a cytoplasmic capping enzyme activity. This function is necessary since all viral RNAs are synthesized in the cytoplasm, and host capping enzymes are restricted to the nucleus. The enzymatic reaction involves a covalent link between 7-methyl-GMP and the methyltransferase, whereas eukaryotic capping enzymes form a covalent complex only with GMP. Methyltransferase catalyzes transfer of a methyl group from S-adenosylmethionine to GTP and GDP to yield m7GTP or m7GDP. GMP, GpppG, and GpppA were poor substrates for the methyltransferase. This enzyme also displays guanylyltransferase activity to form a covalent complex, methyltransferase-m7GMP, from which 7-methyl-GMP is transferred to the mRNA to create the cap structure. Cap analogs such as m7GTP, m7GDP, et2m7GMP, and m2et7GMP inhibit the methyltransferase reaction (By similarity).
Isotype
IgG
Conjugate
Unconjugated
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Modification
Non-structural polyprotein pORF1
Literature
[1]”Recombinant hepatitis E virus genomes infectious for primates: importance of capping and discovery of a cis-reactive element.” Emerson S.U., Zhang M., Meng X.J., Nguyen H., St Claire M., Govindarajan S., Huang Y.K., Purcell R.H. Proc. Natl. Acad. Sci. U.S.A. 98:15270-15275(2001). [2]”Characterization of a prototype strain of hepatitis E virus.” Tsarev S.A., Emerson S.U., Reyes G.R., Tsareva T.S., Legters L.J., Malik I.A., Iqbal M., Purcell R.H. Proc. Natl. Acad. Sci. U.S.A. 89:559-563(1992).
