Description
Aliases
Hemagglutinin
Antibody Type
Polyclonal Antibody
Species
Rotavirus A
Uniprot ID
P11200
Immunogen
Recombinant Rotavirus A Outer capsid protein VP4 protein (247-479AA)
Raised In
Rabbit
Species Reactivity
Rotavirus A
Tested Applications
ELISA;Not yet tested in other applications.
Background / Function
Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. According to the considered strain, VP4 seems to essentially target sialic acid and/or the integrin heterodimer ITGA2/ITGB1 (By similarity).By similarity Outer capsid protein VP5*: forms the spike foot and body. Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm. In integrin-dependent strains, VP5* targets the integrin heterodimer ITGA2/ITGB1 for cell attachment (By similarity).By similarity VP8* forms the head of the spikes. It is the viral hemagglutinin and an important target of neutralizing antibodies. In sialic acid-dependent strains, VP8* binds to host cell sialic acid, most probably a ganglioside, providing the initial contact (By similarity).
Isotype
IgG
Conjugate
Unconjugated
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Modification
Outer capsid protein VP4
Literature
[1]”Conservation of amino acid sequence of VP8 and cleavage region of 84-kDa outer capsid protein among rotaviruses recovered from asymptomatic neonatal infection.” Gorziglia M., Hoshino Y., Buckler-White A., Blumentals I., Glass R., Flores J., Kapikian A.Z., Chanock R.M. Proc. Natl. Acad. Sci. U.S.A. 83:7039-7043(1986). [2]”Group A human rotavirus genomics: evidence that gene constellations are influenced by viral protein interactions.” Heiman E.M., McDonald S.M., Barro M., Taraporewala Z.F., Bar-Magen T., Patton J.T. J. Virol. 82:11106-11116(2008). [3]”Identification of two independent neutralization domains on the VP4 trypsin cleavage products VP5* and VP8* of human rotavirus ST3.” Padilla-Noriega L., Dunn S.J., Lopez S., Greenberg H.B., Arias C.F. Virology 206:148-154(1995).Additional information
| Size | 50μg, 100μg |
|---|
