Aliases
KIP1, MEN4, CDKN4, MEN1B, P27KIP1
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot#: P46527;
NCBI Gene#: 1027;
N
Immunogen
Peptide sequence around phosphorylation site of Threonine 198(R-R-R(p)-Q-T) derived from Human p27 Kip1.
Raised In
Rabbit
Species Reactivity
Human
Tested Applications
WB Recommended dilution: Predicted MW: 27kd, Western blotting: 1:500~1:1000
Background / Function
Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Thr198
