Aliases
DOK1
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q99704
NCBI Protein: NP_001372.1
Immunogen
Peptide sequence around phosphorylation site of tyrosine 362 (P-I-Y(p)-D-E) derived from Human p62Dok.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC Recommended dilution: Predicted MW: 62kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background / Function
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3. Zhou Songyang, et al. (2001) J. Biol. Chem ; 276: 2459 – 2465. Jean-Guy N
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Tyr362
